Selective targeting of striatal parvalbumin-expressing interneurons for transgene delivery

PVCre mice--> combined with AAV-FLEX vectors allowed efficient and specific targeting of PV+ interneurons in the striatum. However, diffusion viral particles to globus pallidus caused massive transduction projection neurons subsequent anterograde transport transgene product subthalamic nucleus substantia nigra pars reticulata. Different AAV serotypes (1 9) promoters (CBA human synapsin) were evaluated. The combination AAV1, a moderate expression level (human synapsin promoter) precise adjustment stereotaxic coordinates anterior dorsolateral part striatum necessary avoid GP neurons. Even absence direct due particles, could be retrogradely transduced via their terminals present dorsal diffusion, GP-Str poorly or not suggesting that retrograde did significantly impair selective striatal Finally, prominent reduction number (about 50 %) was evidenced presence Cre recombinase functional effects AAV-mediated mice should analyzed caution.